Multiplexed optical DNA mapping to identify plasmids and their resistance genes in fecal samples
Paper in proceedings, 2019

Multi-drug resistant bacteria are a major health threat worldwide. Resistance is frequently horizontally transferred among bacteria via mobile genetic elements, such as plasmids. We have developed a one-step staining protocol to reveal sequence-correlated fluorescence profiles along stretched plasmids. In this report, we demonstrate an automation approach to increase the throughput of nanofluidic optical DNA mapping(ODM). Using plasmid samples derived from patients' feces, the results demonstrate an improved throughput, both regarding the number of plasmids analyzed per sample as well as the number of samples analyzed. By combining the ODM with Cas9/CRISPR, plasmids containing the antibiotic resistance gene are readily identified.

Multiplex

Antibiotics resistance

Diagnostics

Optical DNA mapping

Author

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Yii Lih Lin

Chalmers, Biology and Biological Engineering, Chemical Biology

Tsegaye Sewunet

Jimma University

Karolinska Institutet

Shoeib Nematzadeh

Karolinska Institutet

Christian G. Giske

Karolinska Institutet

Karolinska University Hospital

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

856-857

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Subject Categories

Infectious Medicine

Microbiology

Microbiology in the medical area

Areas of Advance

Health Engineering

More information

Latest update

11/13/2020