Multiplexed optical DNA mapping to identify plasmids and their resistance genes in fecal samples

Sriram Kesarimangalam; Yii Lih Lin; Tsegaye Sewunet; Shoeib Nematzadeh; Christian G. Giske; Fredrik Westerlund

Multiplexed optical DNA mapping to identify plasmids and their resistance genes in fecal samples
Paper i proceeding, 2019

Multi-drug resistant bacteria are a major health threat worldwide. Resistance is frequently horizontally transferred among bacteria via mobile genetic elements, such as plasmids. We have developed a one-step staining protocol to reveal sequence-correlated fluorescence profiles along stretched plasmids. In this report, we demonstrate an automation approach to increase the throughput of nanofluidic optical DNA mapping(ODM). Using plasmid samples derived from patients' feces, the results demonstrate an improved throughput, both regarding the number of plasmids analyzed per sample as well as the number of samples analyzed. By combining the ODM with Cas9/CRISPR, plasmids containing the antibiotic resistance gene are readily identified.

Multiplex

Antibiotics resistance

Diagnostics

Optical DNA mapping

Författare

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

Yii Lih Lin

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

Tsegaye Sewunet

Jimma Universitetet

Karolinska Institutet

Shoeib Nematzadeh

Karolinska Institutet

Christian G. Giske

Karolinska Institutet

Karolinska universitetssjukhuset

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

856-857
9781733419000 (ISBN)

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Ämneskategorier

Infektionsmedicin

Mikrobiologi

Mikrobiologi inom det medicinska området

Styrkeområden

Hälsa och teknik

Mer information

Senast uppdaterat

2024-01-03

Multiplexed optical DNA mapping to identify plasmids and their resistance genes in fecal samples Paper i proceeding, 2019

Författare

Sriram Kesarimangalam

Yii Lih Lin

Tsegaye Sewunet

Shoeib Nematzadeh

Christian G. Giske

Fredrik Westerlund

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Ämneskategorier

Styrkeområden

Mer information

Senast uppdaterat

Multiplexed optical DNA mapping to identify plasmids and their resistance genes in fecal samples
Paper i proceeding, 2019