Phosphorylated CtIP bridges DNA to promote annealing of broken ends
Journal article, 2020
The DNA of our cells is constantly exposed to various types of damaging agents. One of the most critical types of damage is when both strands of the DNA break, and thus such breaks need to be efficiently repaired. It is known that CtIP promotes nucleases in DNA break repair. Here we show that CtIP can also hold the two DNA strands together in solution when DNA is free to move, using novel methodology that allows the monitoring of thousands of single DNA molecules in nanofabricated devices. DNA bridging likely facilitates the enzymatic repair steps and identifies novel CtIP functions that are crucial for repairing broken DNA.
Author
Robin Öz
Chalmers, Biology and Biological Engineering, Chemical Biology
Sean Howard
Universita della Svizzera italiana
Rajhans Sharma
Chalmers, Biology and Biological Engineering, Chemical Biology
Hanna Törnkvist
Student at Chalmers
Ilaria Ceppi
Swiss Federal Institute of Technology in Zürich (ETH)
Universita della Svizzera italiana
Sriram Kesarimangalam
Chalmers, Biology and Biological Engineering, Chemical Biology
Erik Kristiansson
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
Petr Cejka
Swiss Federal Institute of Technology in Zürich (ETH)
Universita della Svizzera italiana
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
Proceedings of the National Academy of Sciences of the United States of America
0027-8424 (ISSN) 1091-6490 (eISSN)
Vol. 117 35 21403-21412Areas of Advance
Nanoscience and Nanotechnology
Subject Categories
Cell and Molecular Biology
Biological Sciences
DOI
10.1073/pnas.2008645117
PubMed
32817418