Phosphorylated CtIP bridges DNA to promote annealing of broken ends
Artikel i vetenskaplig tidskrift, 2020
The DNA of our cells is constantly exposed to various types of damaging agents. One of the most critical types of damage is when both strands of the DNA break, and thus such breaks need to be efficiently repaired. It is known that CtIP promotes nucleases in DNA break repair. Here we show that CtIP can also hold the two DNA strands together in solution when DNA is free to move, using novel methodology that allows the monitoring of thousands of single DNA molecules in nanofabricated devices. DNA bridging likely facilitates the enzymatic repair steps and identifies novel CtIP functions that are crucial for repairing broken DNA.
Författare
Robin Öz
Chalmers, Biologi och bioteknik, Kemisk biologi
Sean Howard
Universita della Svizzera italiana
Rajhans Sharma
Chalmers, Biologi och bioteknik, Kemisk biologi
Hanna Törnkvist
Student vid Chalmers
Ilaria Ceppi
Eidgenössische Technische Hochschule Zürich (ETH)
Universita della Svizzera italiana
Sriram Kesarimangalam
Chalmers, Biologi och bioteknik, Kemisk biologi
Erik Kristiansson
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Petr Cejka
Eidgenössische Technische Hochschule Zürich (ETH)
Universita della Svizzera italiana
Fredrik Westerlund
Chalmers, Biologi och bioteknik, Kemisk biologi
Proceedings of the National Academy of Sciences of the United States of America
0027-8424 (ISSN) 1091-6490 (eISSN)
Vol. 117 35 21403-21412Styrkeområden
Nanovetenskap och nanoteknik
Ämneskategorier
Cell- och molekylärbiologi
Biologiska vetenskaper
DOI
10.1073/pnas.2008645117
PubMed
32817418