Transcriptomic response of Saccharomyces cerevisiae to octanoic acid production
Journal article, 2021

The medium-chain fatty acid octanoic acid is an important platform compound widely used in industry. The microbial production from sugars in Saccharomyces cerevisiae is a promising alternative to current non-sustainable production methods, however, titers need to be further increased. To achieve this, it is essential to have in-depth knowledge about the cell physiology during octanoic acid production. To this end, we collected the first RNA-Seq data of an octanoic acid producer strain at three time points during fermentation. The strain produced higher levels of octanoic acid and increased levels of fatty acids of other chain lengths (C6-C18) but showed decreased growth compared to the reference. Furthermore, we show that the here analyzed transcriptomic response to internally produced octanoic acid is notably distinct from a wild type's response to externally supplied octanoic acid as reported in previous publications. By comparing the transcriptomic response of different sampling times, we identified several genes that we subsequently overexpressed and knocked out, respectively. Hereby we identified RPL40B, to date unknown to play a role in fatty acid biosynthesis or medium-chain fatty acid tolerance. Overexpression of RPL40B led to an increase in octanoic acid titers by 40%.

octanoic acid

transcriptome response to medium-chain fatty acids

RNA-Seq

RPL40B

Author

Leonie Baumann

Goethe University Frankfurt

Tyler Doughty

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Verena Siewers

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

E Boles

Goethe University Frankfurt

Mislav Oreb

Goethe University Frankfurt

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 21 2

Subject Categories

Other Basic Medicine

Microbiology

Microbiology in the medical area

DOI

10.1093/femsyr/foab011

PubMed

33599754

More information

Latest update

4/28/2021