Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
Journal article, 2021

A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.

Author

Gaowa Naren

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Wera Larsson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Carlos Benitez-Martin

Centro Andaluz de Nanomedicina y Biotecnologı́a (BIONAND)

University of Malaga

Shiming Li

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Ezequiel Perez-Inestrosa

University of Malaga

Centro Andaluz de Nanomedicina y Biotecnologı́a (BIONAND)

Bo Albinsson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Joakim Andreasson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Chemical Science

2041-6520 (ISSN) 2041-6539 (eISSN)

Vol. 12 20 7073-7078

Subject Categories

Physical Chemistry

Atom and Molecular Physics and Optics

Bioengineering Equipment

DOI

10.1039/d1sc01071a

More information

Latest update

6/28/2021