Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
Artikel i vetenskaplig tidskrift, 2021

A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.

Författare

Gaowa Naren

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Wera Larsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Carlos Benitez-Martin

Centro Andaluz de Nanomedicina y Biotecnologı́a (BIONAND)

Universidad De Malaga

Shiming Li

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Ezequiel Perez-Inestrosa

Centro Andaluz de Nanomedicina y Biotecnologı́a (BIONAND)

Universidad De Malaga

Bo Albinsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Joakim Andreasson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Chemical Science

2041-6520 (ISSN) 2041-6539 (eISSN)

Vol. In Press

Ämneskategorier

Fysikalisk kemi

Atom- och molekylfysik och optik

Bioteknisk apparatteknik

DOI

10.1039/d1sc01071a

Mer information

Senast uppdaterat

2021-05-17