Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
Artikel i vetenskaplig tidskrift, 2021
A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
Författare
Gaowa Naren
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Wera Larsson
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Carlos Benitez-Martin
Centro Andaluz de Nanomedicina y Biotecnologı́a (BIONAND)
Universidad De Malaga
Shiming Li
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Ezequiel Perez-Inestrosa
Universidad De Malaga
Centro Andaluz de Nanomedicina y Biotecnologı́a (BIONAND)
Bo Albinsson
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Joakim Andreasson
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Chemical Science
2041-6520 (ISSN) 2041-6539 (eISSN)
Vol. 12 20 7073-7078Ämneskategorier
Fysikalisk kemi
Atom- och molekylfysik och optik
Bioteknisk apparatteknik
DOI
10.1039/d1sc01071a