Identity of blactx-m carrying plasmids in sequential esbl-e. Coli isolates from patients with recurrent urinary tract infections
Journal article, 2021
Plasmid-mediated multidrug resistance in E. coli is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL E. coli), only five events with a change of ESBL E. coli strain between RUTI episodes were identified. Their blaCTX-M encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar blaCTX-M genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL E. coli strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL E. coli strain reoccurring in both episodes were analyzed. These plasmids (encoding blaCTX-M-3, blaCTX-M-14, and blaCTX-M-15 ) were unaltered for up to six months between recurrent infections. Thus, transmission of blaCTX-M plasmids appears to be a rare event during the course of RUTI. Despite the limited number (n = 23) of plasmids investigated, similar blaCTX-M-15 plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted.
Optical DNA mapping (ODM)
Extended-spectrum beta-lactamase (ESBL)
E. coli
Horizontal transfer
Plasmids
Recurrent urinary tract infection
Author
N. Karami
Sahlgrenska University Hospital
University of Gothenburg
Sriram Kesarimangalam
Chalmers, Biology and Biological Engineering, Chemical Biology
Shora Yazdanshenas
Sahlgrenska University Hospital
University of Gothenburg
Yii Lih Lin
Chalmers, Biology and Biological Engineering, Chemical Biology
Daniel Jaén-Luchoro
University of Gothenburg
Elina Ekedahl
Chalmers, Biology and Biological Engineering, Chemical Biology
Sanjana Parameshwaran
Chalmers, Biology and Biological Engineering, Chemical Biology
Anna Lindblom
Sahlgrenska University Hospital
University of Gothenburg
Christina Åhrén
University of Gothenburg
Region Västra Götaland
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
Microorganisms
20762607 (eISSN)
Vol. 9 6 1138Subject Categories
Infectious Medicine
Microbiology
Microbiology in the medical area
DOI
10.3390/microorganisms9061138
PubMed
34070515