Identity of blactx-m carrying plasmids in sequential esbl-e. Coli isolates from patients with recurrent urinary tract infections
Artikel i vetenskaplig tidskrift, 2021

Plasmid-mediated multidrug resistance in E. coli is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL E. coli), only five events with a change of ESBL E. coli strain between RUTI episodes were identified. Their blaCTX-M encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar blaCTX-M genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL E. coli strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL E. coli strain reoccurring in both episodes were analyzed. These plasmids (encoding blaCTX-M-3, blaCTX-M-14, and blaCTX-M-15 ) were unaltered for up to six months between recurrent infections. Thus, transmission of blaCTX-M plasmids appears to be a rare event during the course of RUTI. Despite the limited number (n = 23) of plasmids investigated, similar blaCTX-M-15 plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted.

Optical DNA mapping (ODM)

Extended-spectrum beta-lactamase (ESBL)

E. coli

Horizontal transfer


Recurrent urinary tract infection


N. Karami

Sahlgrenska universitetssjukhuset

Göteborgs universitet

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Shora Yazdanshenas

Sahlgrenska universitetssjukhuset

Göteborgs universitet

Yii Lih Lin

Chalmers, Biologi och bioteknik, Kemisk biologi

Daniel Jaén-Luchoro

Göteborgs universitet

Elina Ekedahl

Chalmers, Biologi och bioteknik, Kemisk biologi

Sanjana Parameshwaran

Chalmers, Biologi och bioteknik, Kemisk biologi

Anna Lindblom

Sahlgrenska universitetssjukhuset

Göteborgs universitet

Christina Åhrén

Göteborgs universitet

Västra Götalandsregionen

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi


20762607 (eISSN)

Vol. 9 6 1138




Mikrobiologi inom det medicinska området





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