Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism
Journal article, 2022
Objectives Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. Design We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. Results Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration. Conclusion Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
Author
Eugeni Belda
Sorbonne University
Integrative Phenomics
Lise Voland
Sorbonne University
Valentina Tremaroli
University of Gothenburg
Gwen Falony
VIB
KU Leuven
Solia Adriouch
Sorbonne University
Karen E. Assmann
Sorbonne University
Edi Prifiti
Sorbonne University
Judith Aron-Wisnewsky
Sorbonne University
Hôpital de la Salpêtrière
Jean Debedat
Sorbonne University
Tiphaine Le Roy
Sorbonne University
Trine Nielsen
University of Copenhagen
Chloe Amouyal
Sorbonne University
Sebastien Andre
Sorbonne University
Fabrizio Andreelli
Sorbonne University
Matthias Blueher
Leipzig University
Rima Chakaroun
Leipzig University
Julien Chilloux
Imperial College London
Luis Pedro Coelho
European Molecular Biology Laboratory
Maria Carlota Dao
Sorbonne University
Promi Das
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Soraya Fellahi
Sorbonne University
Assistance publique – Hôpitaux de Paris (AP-HP)
Sofia Forslund
The Max Delbrück Center for Molecular Medicine
Nathalie Galleron
University Paris-Saclay
Tue H. Hansen
University of Copenhagen
Bridget Holmes
Danone Nutricia Research
Boyang Ji
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Helle Krogh Pedersen
University of Copenhagen
Phuong Le
Sorbonne University
Emmanuelle Le Chatelier
University Paris-Saclay
Christian Lewinter
Rigshospitalet
Louise Manneras-Holm
University of Gothenburg
Florian Marquet
Sorbonne University
Antonis Myridakis
Imperial College London
Veronique Pelloux
Sorbonne University
Nicolas Pons
University Paris-Saclay
Benoit Quinquis
University Paris-Saclay
Christine Rouault
Sorbonne University
Hugo Roume
University Paris-Saclay
Joe-Elie Salem
Hôpital de la Salpêtrière
Nataliya Sokolovska
Sorbonne University
Nadja B. Sondertoft
University of Copenhagen
Sothea Touch
Sorbonne University
Sara Vieira-Silva
VIB
KU Leuven
Pilar Galan
Université Sorbonne Paris North
Jens Holst
University of Copenhagen
Jens Peter Gotze
Rigshospitalet
Lars Køber
Rigshospitalet
Henrik Vestergaard
Steno Diabetes Center
University of Copenhagen
Torben Hansen
University of Copenhagen
University of Southern Denmark
Serge Hercberg
Université Sorbonne Paris North
Jean-Michel Oppert
Hôpital de la Salpêtrière
Jens B Nielsen
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Ivica Letunic
Biobyte Solutions
Marc-Emmanuel Dumas
Imperial College London
Michael Stumvoll
Leipzig University
Oluf Borbye Pedersen
University of Copenhagen
Peer Bork
European Molecular Biology Laboratory
Stanislav Dusko Ehrlich
King's College London
University Paris-Saclay
Jean-Daniel Zucker
Sorbonne University
Fredrik Baeckhed
University of Gothenburg
Jeroen Raes
KU Leuven
VIB
Karine Clement
Hôpital de la Salpêtrière
Sorbonne University
Gut
0017-5749 (ISSN) 1468-3288 (eISSN)
Vol. 71 12 2463-2480Metagenomics in Cardiometabolic Diseases (METACARDIS)
European Commission (EC) (EC/FP7/305312), 2012-11-01 -- 2017-10-31.
Subject Categories
Endocrinology and Diabetes
Other Clinical Medicine
Microbiology
DOI
10.1136/gutjnl-2021-325753
PubMed
35017197