Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism
Journal article, 2022

Objectives Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. Design We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. Results Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration. Conclusion Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.

Author

Eugeni Belda

Sorbonne University

Integrative Phenomics

Lise Voland

Sorbonne University

Valentina Tremaroli

University of Gothenburg

Gwen Falony

VIB

KU Leuven

Solia Adriouch

Sorbonne University

Karen E. Assmann

Sorbonne University

Edi Prifiti

Sorbonne University

Judith Aron-Wisnewsky

Sorbonne University

Hôpital de la Salpêtrière

Jean Debedat

Sorbonne University

Tiphaine Le Roy

Sorbonne University

Trine Nielsen

University of Copenhagen

Chloe Amouyal

Sorbonne University

Sebastien Andre

Sorbonne University

Fabrizio Andreelli

Sorbonne University

Matthias Blueher

Leipzig University

Rima Chakaroun

Leipzig University

Julien Chilloux

Imperial College London

Luis Pedro Coelho

European Molecular Biology Laboratory

Maria Carlota Dao

Sorbonne University

Promi Das

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Soraya Fellahi

Sorbonne University

Assistance publique – Hôpitaux de Paris (AP-HP)

Sofia Forslund

The Max Delbrück Center for Molecular Medicine

Nathalie Galleron

University Paris-Saclay

Tue H. Hansen

University of Copenhagen

Bridget Holmes

Danone Nutricia Research

Boyang Ji

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Helle Krogh Pedersen

University of Copenhagen

Phuong Le

Sorbonne University

Emmanuelle Le Chatelier

University Paris-Saclay

Christian Lewinter

Rigshospitalet

Louise Manneras-Holm

University of Gothenburg

Florian Marquet

Sorbonne University

Antonis Myridakis

Imperial College London

Veronique Pelloux

Sorbonne University

Nicolas Pons

University Paris-Saclay

Benoit Quinquis

University Paris-Saclay

Christine Rouault

Sorbonne University

Hugo Roume

University Paris-Saclay

Joe-Elie Salem

Hôpital de la Salpêtrière

Nataliya Sokolovska

Sorbonne University

Nadja B. Sondertoft

University of Copenhagen

Sothea Touch

Sorbonne University

Sara Vieira-Silva

VIB

KU Leuven

Pilar Galan

Université Sorbonne Paris North

Jens Holst

University of Copenhagen

Jens Peter Gotze

Rigshospitalet

Lars Køber

Rigshospitalet

Henrik Vestergaard

Steno Diabetes Center

University of Copenhagen

Torben Hansen

University of Copenhagen

University of Southern Denmark

Serge Hercberg

Université Sorbonne Paris North

Jean-Michel Oppert

Hôpital de la Salpêtrière

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Ivica Letunic

Biobyte Solutions

Marc-Emmanuel Dumas

Imperial College London

Michael Stumvoll

Leipzig University

Oluf Borbye Pedersen

University of Copenhagen

Peer Bork

European Molecular Biology Laboratory

Stanislav Dusko Ehrlich

King's College London

University Paris-Saclay

Jean-Daniel Zucker

Sorbonne University

Fredrik Baeckhed

University of Gothenburg

Jeroen Raes

KU Leuven

VIB

Karine Clement

Hôpital de la Salpêtrière

Sorbonne University

Gut

0017-5749 (ISSN) 1468-3288 (eISSN)

Vol. In Press

Metagenomics in Cardiometabolic Diseases (METACARDIS)

European Commission (EC) (EC/FP7/305312), 2012-11-01 -- 2017-10-31.

Subject Categories

Endocrinology and Diabetes

Other Clinical Medicine

Microbiology

DOI

10.1136/gutjnl-2021-325753

PubMed

35017197

More information

Latest update

2/1/2022 8