Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism
Artikel i vetenskaplig tidskrift, 2022
Objectives Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. Design We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. Results Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration. Conclusion Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
Författare
Eugeni Belda
Sorbonne Université
Integrative Phenomics
Lise Voland
Sorbonne Université
Valentina Tremaroli
Göteborgs universitet
Gwen Falony
VIB
KU Leuven
Solia Adriouch
Sorbonne Université
Karen E. Assmann
Sorbonne Université
Edi Prifiti
Sorbonne Université
Judith Aron-Wisnewsky
Sorbonne Université
Hôpital de la Salpêtrière
Jean Debedat
Sorbonne Université
Tiphaine Le Roy
Sorbonne Université
Trine Nielsen
Köpenhamns universitet
Chloe Amouyal
Sorbonne Université
Sebastien Andre
Sorbonne Université
Fabrizio Andreelli
Sorbonne Université
Matthias Blueher
Universität Leipzig
Rima Chakaroun
Universität Leipzig
Julien Chilloux
Imperial College London
Luis Pedro Coelho
European Molecular Biology Laboratory
Maria Carlota Dao
Sorbonne Université
Promi Das
Chalmers, Biologi och bioteknik, Systembiologi
Soraya Fellahi
Sorbonne Université
Assistance publique – Hôpitaux de Paris (AP-HP)
Sofia Forslund
Max Delbrueck Centrum Fuer Molekulare Medizin
Nathalie Galleron
Université Paris-Saclay
Tue H. Hansen
Köpenhamns universitet
Bridget Holmes
Danone Nutricia Research
Boyang Ji
Chalmers, Biologi och bioteknik, Systembiologi
Helle Krogh Pedersen
Köpenhamns universitet
Phuong Le
Sorbonne Université
Emmanuelle Le Chatelier
Université Paris-Saclay
Christian Lewinter
Rigshospitalet
Louise Manneras-Holm
Göteborgs universitet
Florian Marquet
Sorbonne Université
Antonis Myridakis
Imperial College London
Veronique Pelloux
Sorbonne Université
Nicolas Pons
Université Paris-Saclay
Benoit Quinquis
Université Paris-Saclay
Christine Rouault
Sorbonne Université
Hugo Roume
Université Paris-Saclay
Joe-Elie Salem
Hôpital de la Salpêtrière
Nataliya Sokolovska
Sorbonne Université
Nadja B. Sondertoft
Köpenhamns universitet
Sothea Touch
Sorbonne Université
Sara Vieira-Silva
VIB
KU Leuven
Pilar Galan
Université Sorbonne Paris North
Jens Holst
Köpenhamns universitet
Jens Peter Gotze
Rigshospitalet
Lars Køber
Rigshospitalet
Henrik Vestergaard
Steno Diabetes Center
Köpenhamns universitet
Torben Hansen
Köpenhamns universitet
Syddansk Universitet
Serge Hercberg
Université Sorbonne Paris North
Jean-Michel Oppert
Hôpital de la Salpêtrière
Jens B Nielsen
Chalmers, Biologi och bioteknik, Systembiologi
Ivica Letunic
Biobyte Solutions
Marc-Emmanuel Dumas
Imperial College London
Michael Stumvoll
Universität Leipzig
Oluf Borbye Pedersen
Köpenhamns universitet
Peer Bork
European Molecular Biology Laboratory
Stanislav Dusko Ehrlich
King's College London
Université Paris-Saclay
Jean-Daniel Zucker
Sorbonne Université
Fredrik Baeckhed
Göteborgs universitet
Jeroen Raes
KU Leuven
VIB
Karine Clement
Hôpital de la Salpêtrière
Sorbonne Université
Gut
0017-5749 (ISSN) 1468-3288 (eISSN)
Vol. 71 12 2463-2480Metagenomics in Cardiometabolic Diseases (METACARDIS)
Europeiska kommissionen (EU) (EC/FP7/305312), 2012-11-01 -- 2017-10-31.
Ämneskategorier
Endokrinologi och diabetes
Annan klinisk medicin
Mikrobiologi
DOI
10.1136/gutjnl-2021-325753
PubMed
35017197