Robustness: linking strain design to viable bioprocesses
Review article, 2022

Microbial cell factories are becoming increasingly popular for the sustainable production of various chemicals. Metabolic engineering has led to the design of advanced cell factories; however, their long-term yield, titer, and productivity falter when scaled up and subjected to industrial conditions. This limitation arises from a lack of robustness – the ability to maintain a constant phenotype despite the perturbations of such processes. This review describes predictable and stochastic industrial perturbations as well as state-of-the-art technologies to counter process variability. Moreover, we distinguish robustness from tolerance and discuss the potential of single-cell studies for improving system robustness. Finally, we highlight ways of achieving consistent and comparable quantification of robustness that can guide the selection of strains for industrial bioprocesses.

industrial production

biosensors

single-cell analysis

robustness quantification

bioprocess engineering

perturbations

Author

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Peter Rugbjerg

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Enduro Genetics ApS

Luca Torello Pianale

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Cecilia Trivellin

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Trends in Biotechnology

0167-7799 (ISSN) 18793096 (eISSN)

Vol. 40 8 918-931

Subject Categories

Chemical Process Engineering

Bioprocess Technology

DOI

10.1016/j.tibtech.2022.01.004

PubMed

35120750

More information

Latest update

3/7/2024 9