Robustness: linking strain design to viable bioprocesses
Reviewartikel, 2022

Microbial cell factories are becoming increasingly popular for the sustainable production of various chemicals. Metabolic engineering has led to the design of advanced cell factories; however, their long-term yield, titer, and productivity falter when scaled up and subjected to industrial conditions. This limitation arises from a lack of robustness – the ability to maintain a constant phenotype despite the perturbations of such processes. This review describes predictable and stochastic industrial perturbations as well as state-of-the-art technologies to counter process variability. Moreover, we distinguish robustness from tolerance and discuss the potential of single-cell studies for improving system robustness. Finally, we highlight ways of achieving consistent and comparable quantification of robustness that can guide the selection of strains for industrial bioprocesses.

industrial production

biosensors

single-cell analysis

robustness quantification

bioprocess engineering

perturbations

Författare

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Peter Rugbjerg

Chalmers, Biologi och bioteknik, Industriell bioteknik

Enduro Genetics ApS

Luca Torello Pianale

Chalmers, Biologi och bioteknik, Industriell bioteknik

Cecilia Trivellin

Chalmers, Biologi och bioteknik, Industriell bioteknik

Trends in Biotechnology

0167-7799 (ISSN)

Vol. In Press

Ämneskategorier

Kemiska processer

Bioprocessteknik

DOI

10.1016/j.tibtech.2022.01.004

Mer information

Senast uppdaterat

2022-02-10