CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS
Paper in proceeding, 2021

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.

Plasmid

Single-molecule

DNA combing

DNA

Anti-microbial resistance

CRISPR/Cas9

Author

Gaurav Goyal

Chalmers, Biology and Biological Engineering, Chemical Biology

Other publications Research

Elina Ekedahl

Chalmers, Biology and Biological Engineering, Chemical Biology

My Nyblom

Chalmers, Biology and Biological Engineering, Chemical Biology

Other publications Research

Jens Krog

Lund University

Erik Torstensson

Lund University

Tsegaye Sewunet

Karolinska Institutet

Christian G. Giske

Karolinska Institutet

Visanu Thamlikitkul

Siriraj Hospital

Tobias Ambjörnsson

Lund University

Other publications Research

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Other publications Research

MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

801-802
9781733419031 (ISBN)

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Palm Springs, Virtual, USA,

Subject Categories

Infectious Medicine

Microbiology

Microbiology in the medical area

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Latest update

10/23/2023

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