CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS
Paper i proceeding, 2021

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.

Plasmid

Single-molecule

DNA combing

DNA

Anti-microbial resistance

CRISPR/Cas9

Författare

Gaurav Goyal

Chalmers, Biologi och bioteknik, Kemisk biologi

Elina Ekedahl

Chalmers, Biologi och bioteknik, Kemisk biologi

My Nyblom

Chalmers, Biologi och bioteknik, Kemisk biologi

Jens Krog

Lunds universitet

Erik Torstensson

Lunds universitet

Tsegaye Sewunet

Karolinska Institutet

Christian G. Giske

Karolinska Institutet

Visanu Thamlikitkul

Siriraj Hospital

Tobias Ambjörnsson

Lunds universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

801-802
9781733419031 (ISBN)

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Palm Springs, Virtual, USA,

Ämneskategorier

Infektionsmedicin

Mikrobiologi

Mikrobiologi inom det medicinska området

Mer information

Senast uppdaterat

2023-10-23