Untargeted Metabolomics Based on Liquid Chromatography-Mass Spectrometry for the Analysis of Plasma and Erythrocyte Samples in Childhood Obesity
Book chapter, 2023

The circulating metabolome of human peripheral blood provides valuable information to investigate the molecular mechanisms underlying the development of diseases and to discover candidate biomarkers. In particular, erythrocytes have been proposed as potential systemic indicators of the metabolic and redox status of the organism. To accomplish wide-coverage metabolomics analysis, the combination of complementary analytical techniques is necessary to manage the physicochemical complexity of the human metabolome. Herein, we describe an untargeted metabolomics method to capture the plasmatic and erythroid metabolomes based on ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry, combining reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography. The method provides comprehensive metabolomics fingerprinting of plasma and erythrocyte samples, thereby enabling the elucidation of the distinctive metabolic disturbances behind childhood obesity and associated comorbidities, such as insulin resistance.

Childhood obesity


Mass spectrometry

Liquid chromatography




Álvaro González-Domínguez

Hospital Universitario Puerta del Mar

Marina Armeni

Chalmers, Life Sciences, Systems and Synthetic Biology

Otto Savolainen

Chalmers, Life Sciences, Systems and Synthetic Biology

Alfonso María Lechuga-Sancho

Hospital Universitario Puerta del Mar

Universidad de Cadiz, Facultad de Medicina

Rikard Landberg

Chalmers, Life Sciences, Food and Nutrition Science

Raúl González-Domínguez

Hospital Universitario Puerta del Mar

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)


Subject Categories

Pharmaceutical Sciences

Analytical Chemistry

Biomedical Laboratory Science/Technology


Chalmers Infrastructure for Mass spectrometry





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