Untargeted Metabolomics Based on Liquid Chromatography-Mass Spectrometry for the Analysis of Plasma and Erythrocyte Samples in Childhood Obesity
Kapitel i bok, 2023

The circulating metabolome of human peripheral blood provides valuable information to investigate the molecular mechanisms underlying the development of diseases and to discover candidate biomarkers. In particular, erythrocytes have been proposed as potential systemic indicators of the metabolic and redox status of the organism. To accomplish wide-coverage metabolomics analysis, the combination of complementary analytical techniques is necessary to manage the physicochemical complexity of the human metabolome. Herein, we describe an untargeted metabolomics method to capture the plasmatic and erythroid metabolomes based on ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry, combining reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography. The method provides comprehensive metabolomics fingerprinting of plasma and erythrocyte samples, thereby enabling the elucidation of the distinctive metabolic disturbances behind childhood obesity and associated comorbidities, such as insulin resistance.

Childhood obesity

Plasma

Mass spectrometry

Liquid chromatography

Metabolomics

Erythrocyte

Författare

Álvaro González-Domínguez

Hospital Universitario Puerta del Mar

Marina Armeni

Chalmers, Life sciences, Systembiologi

Otto Savolainen

Chalmers, Life sciences, Systembiologi

Alfonso María Lechuga-Sancho

Hospital Universitario Puerta del Mar

Universidad de Cadiz, Facultad de Medicina

Rikard Landberg

Chalmers, Life sciences, Livsmedelsvetenskap

Raúl González-Domínguez

Hospital Universitario Puerta del Mar

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)

Vol. 2571 115-122

Ämneskategorier

Farmaceutisk vetenskap

Analytisk kemi

Biomedicinsk laboratorievetenskap/teknologi

Infrastruktur

Chalmers infrastruktur för masspektrometri

DOI

10.1007/978-1-0716-2699-3_11

PubMed

36152155

Mer information

Senast uppdaterat

2024-07-12