Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms
Review article, 2024
Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic changes has become an increasingly attractive approach for constructing robust microbial cell factories. In this review, we provide a brief overview of metabolic burden engineering, focusing specifically on recent developments and strategies for diminishing the burden while improving robustness and yield. A variety of examples are presented to showcase the promise of metabolic burden engineering in facilitating the design and construction of robust microbial cell factories. Finally, challenges and limitations encountered in metabolic burden engineering are discussed.
Physiological engineering
Constrained models
Dynamic control
Microbial cell factories
Microbial consortia
Author
Jiwei Mao
Chalmers, Life Sciences, Systems and Synthetic Biology
Hongyu Zhang
Chinese Academy of Sciences
Tianjin Institute of Industrial Biotechnology
Yu Chen
Shenzhen Institute of Advanced Technology
Liang Wei
Tianjin Institute of Industrial Biotechnology
Jun Liu
Tianjin Institute of Industrial Biotechnology
Jens B Nielsen
Chalmers, Life Sciences, Systems and Synthetic Biology
BioInnovation Institute
Yun Chen
Chalmers, Life Sciences, Systems and Synthetic Biology
Novo Nordisk Foundation
Ning Xu
Tianjin Institute of Industrial Biotechnology
Chinese Academy of Sciences
Biotechnology Advances
0734-9750 (ISSN)
Vol. 74 108401Direct fermentation route for sustainable plastics and superabsorbent polymers
Formas (2022-01130), 2023-01-01 -- 2025-12-31.
Subject Categories
Microbiology
Microbiology in the medical area
DOI
10.1016/j.biotechadv.2024.108401