Metabolite Biomarkers Linking a High-Fiber Rye Intervention with Cardiometabolic Risk Factors: The RyeWeight Study
Journal article, 2025
Wholegrain rye, considered one of the cereals with the highest content of dietary fiber and bioactive compounds, has been linked with reduced risk of cardiometabolic diseases. Thus, biomarkers reflecting its intake and/or the metabolic effect after consumption are essential to better elucidate its health effects. Our aim was to identify plasma metabolite biomarkers associated with a high-fiber rye intervention and to assess the associations between these metabolites, gut microbiota composition, and cardiometabolic risk factors in a 12-week randomized controlled trial comparing a hypocaloric diet with high-fiber rye (n = 108) or refined wheat (n = 99) in participants with obesity. Rye intervention increased plasma concentrations of benzoxazinoids (DIBOA-S) and phenylacetamides (2-HPA-S and 2-HHPA-S), gut microbial metabolites (indolepropionic acid, 2-aminophenol, enterolactone sulfate, and enterolactone glucuronide), betainized compounds (pipecolic-betaine), phenolic acids (2,6-DHBA and gallic acid-4-sulfate), and diverse endogenous metabolites. Microbiota composition changes were increased Eubacterium xylanophilum and Agathobacter and decreased Ruminococcus torques and Romboutsia. Moreover, the intervention effect was mostly captured by changes in metabolites and gut microbiota compared to clinical variables. Gallic acid-4-sulfate and phenylacetamides were associated with reductions in weight, fat mass, BMI, or fasting insulin levels even after adjusting for plasma alkylresorcinols, used as markers for rye intake compliance. Altogether, these metabolites may constitute biomarkers of wholegrain rye cardiometabolic effects.
cardiometabolic health
metabolomics
wholegrain rye
randomized controlled trial
gutmicrobiota
biomarker
refined wheat
Author
Andrea Union Caballero
University of Barcelona
Institute of Health Carlos III
Tomas Merono
Institute of Health Carlos III
University of Barcelona
Sebastian Åberg
Chalmers, Life Sciences, Food and Nutrition Science
Elise Nordin
Chalmers, Life Sciences, Food and Nutrition Science
Johan Dicksved
Uppsala University
Alex Sanchez-Pla
University of Barcelona
Institute of Health Carlos III
Marta Cubedo
University of Barcelona
Institute of Health Carlos III
Francisco Carmona-Pontaque
Institute of Health Carlos III
University of Barcelona
Kia Noehr Iversen
Chalmers, Life Sciences, Food and Nutrition Science
Miriam Martinez-Huelamo
Institute of Health Carlos III
University of Barcelona
Anna Guadall
University of Barcelona
Institute of Health Carlos III
Rikard Landberg
Chalmers, Life Sciences, Food and Nutrition Science
Cristina Andres-Lacueva
University of Barcelona
Institute of Health Carlos III
Journal of Agricultural and Food Chemistry
0021-8561 (ISSN) 1520-5118 (eISSN)
Vol. 73 35 21869-21879Discovery and valdiation of novel biomarkers of gut microbiota, diet and their interactions associated with type 2 diabetes risk
Swedish Research Council (VR) (2019-01264), 2020-01-01 -- 2022-12-31.
Subject Categories (SSIF 2025)
Food Science
Nutrition and Dietetics
DOI
10.1021/acs.jafc.5c01415
PubMed
40838659