In vitro digestive stability of complexes between gliadin and synthetic blocking peptides
Journal article, 2011

Celiac disease is caused by an inappropriate immune response to incompletely digested gluten proteins. We investigated whether synthetic peptides with high affinity to wheat gliadin could be selected with a phage display technique and whether complexes between such peptides and gliadin could sustain gastric and pancreatic digestion. Two synthetic peptides, P61 and P64, were selected because of their high affinity to immobilized gliadin. They were allowed to form complexes with gliadin, whereafter the complexes were subjected to in vitro digestion with gastric and pancreatic enzymes. The digestion products were analyzed with Western blot and RP HPLC. The results showed that both peptides formed stable complexes with intact gliadin and that complexes between gliadin and peptide P64 partly resisted gastrointestinal digestion. The two peptides reduced the binding of serum anti-gliadin IgA antibodies by 12%, and 11.5%, respectively, and the binding of anti-gliadin antibodies of the IgG isotype by 13% and 10%. Thus peptides produced by a phage display technique could interact stably with gliadin partly masking epitopes for antibody binding. A combination of peptides of this kind may be used to block gliadin-immune system interactions.

allergens

RP

in vitro digestion

gliadin

proteins

celiac disease

HPLC/immunochemistry

cells

celiac-disease

blocking peptides

Author

Karolina Hoffmann

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Nils-Gunnar Carlsson

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Marie Alminger

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Tingsu Chen

University of Gothenburg

Agnes E Wold

University of Gothenburg

Olof Olsson

University of Gothenburg

Ann-Sofie Sandberg

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Biotechnology and Applied Biochemistry

0885-4513 (ISSN) 1470-8744 (eISSN)

Vol. 58 3 190-197

Subject Categories

Biochemistry and Molecular Biology

Immunology in the medical area

Other Industrial Biotechnology

Areas of Advance

Life Science Engineering (2010-2018)

More information

Created

10/7/2017