Engineering of acetyl-CoA metabolism for the improved production of polyhydroxybutyrate in Saccharomyces cerevisiae
Journal article, 2012

Through metabolic engineering microorganisms can be engineered to produce new products and further produce these with higher yield and productivities. Here, we expressed the bacterial polyhydroxybutyrate (PHB) pathway in the yeast Saccharomyces cerevisiae and we further evaluated the effect of engineering the formation of acetyl coenzyme A (acetyl-CoA), an intermediate of the central carbon metabolism and precursor of the PHB pathway, on heterologous PHB production by yeast. We engineered the acetyl-CoA metabolism by co-transformation of a plasmid containing genes for native S. cerevisiae alcohol dehydrogenase (ADH2), acetaldehyde dehydrogenase (ALD6), acetyl-CoA acetyltransferase (ERG10) and a Salmonella enterica acetyl-CoA synthetase variant (acsL641P), resulting in acetoacetyl-CoA overproduction, together with a plasmid containing the PHB pathway genes coding for acetyl-CoA acetyltransferase (phaA), NADPH-linked acetoacetyl-CoA reductase (phaB) and poly(3-hydroxybutyrate) polymerase (phaC) from Ralstonia eutropha H16. Introduction of the acetyl-CoA plasmid together with the PHB plasmid, improved the productivity of PHB more than 16 times compared to the reference strain used in this study, as well as it reduced the specific product formation of side products.

Pathway engineering


Saccharomyces cerevisiae

Acetyl coenzyme A


Kanokarn Kocharin

Chalmers, Chemical and Biological Engineering, Life Sciences

Yun Chen

Chalmers, Chemical and Biological Engineering, Life Sciences

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences

AMB Express

21910855 (eISSN)

Vol. 2 1 52

Subject Categories

Biological Sciences

Areas of Advance

Life Science Engineering (2010-2018)



More information

Latest update

4/5/2022 6