Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism
Journal article, 2013

Production of fuels and chemicals by industrial biotechnology requires efficient, safe and flexible cell factory platforms that can be used for production of a wide range of compounds. Here we developed a platform yeast cell factory for efficient provision of acetyl-CoA that serves as precursor metabolite for a wide range of industrially interesting products. We demonstrate that the platform cell factory can be used to improve the production of alpha-santalene, a plant sesquiterpene that can be used as a perfume by four-fold. This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products.

Acetyl-CoA

Saccharomyces cerevisiae

saccharomyces-cerevisiae

pyruvate-decarboxylase

expression

Cell factory

growth-rate

precursor

synthetase

alpha-Santalene

sequence

glucose

transcription

fermentation

Author

Yun Chen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

L. Daviet

Firmenich Inc.

M. Schalk

Firmenich Inc.

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 15 1 48-54

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

European Commission (FP7), 2010-01-01 -- 2014-12-31.

Driving Forces

Sustainable development

Subject Categories

Biological Sciences

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.1016/j.ymben.2012.11.002

More information

Latest update

9/6/2018 1