Comparative Systems Analyses Reveal Molecular Signatures of Clinically tested Vaccine Adjuvants
Journal article, 2016

A better understanding of the mechanisms of action of human adjuvants could inform a rational development of next generation vaccines for human use. Here, we exploited a genome wide transcriptomics analysis combined with a systems biology approach to determine the molecular signatures induced by four clinically tested vaccine adjuvants, namely CAF01, IC31, GLA-SE and Alum in mice. We report signature molecules, pathways, gene modules and networks, which are shared by or otherwise exclusive to these clinical-grade adjuvants in whole blood and draining lymph nodes of mice. Intriguingly, co-expression analysis revealed blood gene modules highly enriched for molecules with documented roles in T follicular helper (TFH) and germinal center (GC) responses. We could show that all adjuvants enhanced, although with different magnitude and kinetics, TFH and GC B cell responses in draining lymph nodes. These results represent, to our knowledge, the first comparative systems analysis of clinically tested vaccine adjuvants that may provide new insights into the mechanisms of action of human adjuvants.

t-cell responses

immunity

Science & Technology - Other Topics

activation

dendritic cells

alum adjuvant

generation

analysis

tuberculosis vaccine

expression

infection

network

Author

T. A. Olafsdottir

University of Gothenburg

M. Lindqvist

University of Gothenburg

Intawat Nookaew

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

P. Andersen

Statens Serum Institut

J. Maertzdorf

Max Planck Institute

J. Persson

University of Gothenburg

D. Christensen

Statens Serum Institut

Y. Zhang

University of Gothenburg

J. Anderson

University of Gothenburg

Sakda Khoomrung

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Partho Sen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

E. M. Agger

Statens Serum Institut

R. Coler

Infectious Disease Research Institute

D. Carter

Infectious Disease Research Institute

A. Meinke

Vienna Biocenter

R. Rappuoli

GSK Vaccines

S. H. E. Kaufmann

Max Planck Institute

S. G. Reed

Infectious Disease Research Institute

A. M. Harandi

University of Gothenburg

Scientific Reports

2045-2322 (ISSN)

Vol. 6 39097

Subject Categories

Biocatalysis and Enzyme Technology

Organic Chemistry

DOI

10.1038/srep39097

More information

Latest update

9/6/2018 2