Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri
Journal article, 2021

Background Limosilactobacillus reuteri (earlier known as Lactobacillus reuteri) is a well-studied lactic acid bacterium, with some specific strains used as probiotics, that exists in different hosts such as human, pig, goat, mouse and rat, with multiple body sites such as the gastrointestinal tract, breast milk and mouth. Numerous studies have confirmed the beneficial effects of orally administered specific L. reuteri strains, such as preventing bone loss and promoting regulatory immune system development. L. reuteri ATCC PTA 6475 is a widely used strain that has been applied in the market as a probiotic due to its positive effects on the human host. Its health benefits may be due, in part, to the production of beneficial metabolites. Considering the strain-specific effects and genetic diversity of L. reuteri strains, we were interested to study the metabolic versatility of these strains. Results In this study, we aimed to systematically investigate the metabolic features and diversities of L. reuteri strains by using genome-scale metabolic models (GEMs). The GEM of L. reuteri ATCC PTA 6475 was reconstructed with a template-based method and curated manually. The final GEM iHL622 of L. reuteri ATCC PTA 6475 contains 894 reactions and 726 metabolites linked to 622 metabolic genes, which can be used to simulate growth and amino acids utilization. Furthermore, we built GEMs for the other 35 L. reuteri strains from three types of hosts. The comparison of the L. reuteri GEMs identified potential metabolic products linked to the adaptation to the host. Conclusions The GEM of L. reuteri ATCC PTA 6475 can be used to simulate metabolic capabilities and growth. The core and pan model of 35 L. reuteri strains shows metabolic capacity differences both between and within the host groups. The GEMs provide a reliable basis to investigate the metabolism of L. reuteri in detail and their potential benefits on the host.

Lactobacillus

Genome-scale metabolic model

Metabolic versatility

Limosilactobacillus reuteri

Probiotic

Pan metabolism

Core metabolism

Author

Hao Luo

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Peishun Li

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Hao Wang

University of Gothenburg

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology, CSBI

Stefan Roos

Swedish University of Agricultural Sciences (SLU)

Boyang Ji

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

BioInnovation Institute

BMC Biotechnology

1472-6750 (ISSN)

Vol. 21 1 46

Subject Categories

Medical Biotechnology

Microbiology

Microbiology in the medical area

DOI

10.1186/s12896-021-00702-w

PubMed

34330235

More information

Latest update

8/19/2021