Development of an Haa1-Based Biosensor for Acetic Acid Sensing in Saccharomyces cerevisiae
Book chapter, 2024

Transcription factor (TF)-based biosensors are important tools in strain development and screening as they can allow accurate monitoring of intracellular concentrations of a molecule. Acetic acid is one of the main inhibitors in lignocellulosic biomass and a major challenge when using yeast cell factories for biorefinery applications. Thus, developing acetic acid tolerant strains is of great importance. The acetic acid sensing biosensor developed relies on the endogenous Saccharomyces cerevisiae TF Haa1 that upon binding of acetic acid translocates to the nucleus. The acetic acid biosensor can be used as a tool for strain development and evaluation, as well as for screening of acetic acid-producing strains and for dynamic monitoring of acetic acid accumulation. This chapter describes a methodology for developing a TF-based biosensor for acetic acid sensing. Protocols for design considerations, part construction, and characterization procedures are included. The approach can potentially be adapted to any molecule where a suitable TF can be identified.

Synthetic transcription factor

Acetic acid

Haa1

Synthetic promoter

Biosensor

Author

Elin Blick

Chalmers, Life Sciences, Industrial Biotechnology

Maurizio Mormino

Chalmers, Life Sciences, Industrial Biotechnology

Verena Siewers

Chalmers, Life Sciences, Systems and Synthetic Biology

Novo Nordisk Foundation

Yvonne Nygård

Technical Research Centre of Finland (VTT)

Chalmers, Life Sciences, Industrial Biotechnology

Methods in Molecular Biology

Vol. 2844 221-238

Subject Categories

Analytical Chemistry

Other Industrial Biotechnology

DOI

10.1007/978-1-0716-4063-0_15

PubMed

39068343

More information

Latest update

8/20/2024