CARS and SHG microscopy of artificial bioengineered tissues
Konferensbidrag (offentliggjort, men ej förlagsutgivet), 2010

Major efforts are presently made to develop artificial replacement tissues with optimal architectural and material characteristics, mimicking those of their natural correspondents. Encouraged by the readiness with which cellulose fibers woven by the bacteria Acetobacter xylinum can be formed into organ-like macroscopic shapes and with different microscopic textures, it emerges as an interesting material within tissue engineering. We have developed a protocol employing simultaneous CARS and SHG microscopy for monitoring the cellulose network characteristics and its impact on the integration of smooth muscle cells (SMCs) for functionalized artificial tissues. CARS and SHG overlay images of the cells and the cellulose fibers reveal an immediate interaction irrespective of scaffold morphology and that the SMCs attach to the cellulose fibers already during the first cultivation day without cell-adhesive coatings. During the subsequent 28 days, SMCs were found to readily proliferate and differentiate on the cellulose scaffold without the need for exogenous growth factors. However, the efficiency with which this occurred depended on the topography of the cellulose constructs, benefited by porous and less compact matrices. This brings forward the need for in-depth studies on how the microstructure of tissue scaffolds influences and can be optimized for native cell integration and proliferation, studies where the benefits of multi-modal non-linear microscopy can be fully exploited. © 2010 Copyright SPIE - The International Society for Optical Engineering.

Artificial tissues

Macroscopic shapes

Smooth muscle cells

Functionalized

Exogenous growth

In-depth study

Cell integration

Multi-modal

Adhesive coatings

Nonlinear microscopy

Cellulose fiber

Tissue scaffolds

Network characteristics

Scaffold morphology

Bioengineered tissue

Material characteristics

Acetobacter xylinum

Författare

Annika Enejder

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Christian Brackmann

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Jan-Olof Dahlberg

Chalmers University of Technology

E. Vrana

Chalmers University of Technology

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

1605-7422 (ISSN)

Vol. 7569

Ämneskategorier

Kemi

DOI

10.1117/12.841251

ISBN

978-081947965-5