Effect of microwave radiation on permeability of liposomes. Evidence against non-thermal leakage
Artikel i vetenskaplig tidskrift, 1994

The effect of 2.45 GHz microwave radiation on the permeability of unilamellar phosphatidylcholine liposomes has been studied. Leakage of 5(6)-calboxyfluorescein from the liposomes was measured using spectrofluorimetry after exposure to either microwaves or thermal heating for 5-20 min intervals. The exposure temperature, 37.6 +/- 0.5 degrees C, was well above the phase transition temperature of the lipid membrane. The microwave exposure did not result in any non-thermal increase in permeability above that produced by thermal heating. This study refutes the results reported by Saalman et al. [1] in which an increased liposome permeability due to microwave exposure was reported. The refined analysis in the present study shows that this increased liposome permeability was not a non-thermal microwave effect.

microwave radiation

liposome

nonthermal effect

phase-transition

fluorescence

5(6)-carboxyfluorescein

temperature

membrane permeability

erythrocytes

efflux

Författare

Björn Bergqvist

Institutionen för mikrovågsteknik

Lars Arvidsson

Institutionen för biokemi och biofysik

Eva Pettersson

Institutionen för biokemi och biofysik

Sheila Galt

Institutionen för mikrovågsteknik

Elisabeth Saalman

Yngve Hamnerius

Institutionen för mikrovågsteknik

Bengt Nordén

Institutionen för fysikalisk kemi

Biochimica et Biophysica Acta - General Subjects

0304-4165 (ISSN)

Vol. 1201 51-54

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/0304-4165(94)90150-3