Continuous Lipid Bilayers Derived from Cell Membranes for Spatial Molecular Manipulation
Artikel i vetenskaplig tidskrift, 2011

Progress with respect to enrichment and separation of native membrane components in complex lipid environments, such as native cell membranes, has so far been very limited. The reason for the slow progress can be related to the lack of efficient means to generate continuous and laterally fluid supported lipid bilayers (SLBs) made from real cell membranes. We show in this work how the edge of a hydrodynamically driven SLB can be used to induce rupture of adsorbed lipid vesicles of compositions that typically prevent spontaneous SLB formation, such as vesicles made of complex lipid compositions, containing high cholesterol content or being derived from real cell membranes. In particular, upon fusion between the moving edge of a preformed SLB and adsorbed vesicles made directly from 3T3 fibroblast cell membranes, the membrane content of the vesicles was shown to be efficiently transferred to the SLB. The molecular transfer was verified using cholera toxin B subunit (CTB) binding to monosialoganglioside receptors (G(M1) and G(M3)), and the preserved lateral mobility was confirmed by spatial manipulation of the G(M1/M3)-CTB complex using a hydrodynamic flow. Two populations of CTB with markedly different drift velocity could be identified, which from dissociation kinetics data were attributed to CTB bound with different numbers of ganglioside anchors.

proteins

cholesterol

vesicle adsorption

model membranes

separation

receptor

surfaces

quartz-crystal microbalance

cholera-toxin

immobilization

Författare

Lisa Simonsson Nyström

Chalmers, Teknisk fysik, Biologisk fysik

Anders Gunnarsson

Chalmers, Teknisk fysik, Biologisk fysik

Patric Wallin

Chalmers, Teknisk fysik, Biologisk fysik

Peter Jönsson

Chalmers, Teknisk fysik, Biologisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 133 14027-14032

Ämneskategorier

Kemi

DOI

10.1021/ja204589a