Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids
Artikel i vetenskaplig tidskrift, 2011

Glycosphingolipids are involved in the first steps of virus-cell interaction, where they mediate specific recognition of the host cell membrane. We have employed total-internal-reflection fluorescence microscopy to explore the interaction kinetics between individual unlabeled noroviruslike particles, which are attached to a glycosphingolipid-containing lipid bilayer, and fluorescent vesicles containing different types and concentrations of glycosphingolipids. Under association equilibrium, the vesicle-binding rate is found to be kinetically limited, yielding information on the corresponding activation energy. The dissociation kinetics are logarithmic over a wide range of time. The latter is explained by the vesicle-size-related distribution of the dissociation activation energy. The biological, pharmaceutical, and diagnostic relevance of the study is briefly discussed.

mismatch discrimination

tracking

cells

virus

norwalk

noroviruses

infection pathway

entry

binding

bilayers

influenza-viruses

Författare

Marta Bally

Chalmers, Teknisk fysik, Biologisk fysik

Anders Gunnarsson

Chalmers, Teknisk fysik, Biologisk fysik

L. Svensson

Göran Larson

Göteborgs universitet

Vladimir Zhdanov

Chalmers, Teknisk fysik, Kemisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Physical Review Letters

0031-9007 (ISSN) 1079-7114 (eISSN)

Vol. 107

Ämneskategorier

Fysikalisk kemi

Fysik

Klinisk laboratoriemedicin

Nanoteknik

Mikrobiologi inom det medicinska området

DOI

10.1103/PhysRevLett.107.188103

PubMed

22107678