A single-step competitive binding assay for mapping of single DNA molecules
Artikel i vetenskaplig tidskrift, 2012

Optical mapping of genomic DNA is of relevance for a plethora of applications such as scaffolding for sequencing and detection of structural variations as well as identification cif pathogens like bacteria and viruses. For future clinical applications it is desirable to have a fast and robust mapping method based on as few steps as possible. We here demonstrate a single-step method to obtain a DNA barcode that is directly visualized using nanofluidic devices and fluorescence microscopy. Using a mixture of YOYO-1, a bright DNA dye, and netropsin, a natural antibiotic with very high AT specificity, we obtain a DNA map with a fluorescence intensity profile along the DNA that reflects the underlying sequence. The netropsin binds to AT-tetrads and blocks these binding sites from YOYO-1 binding which results in lower fluorescence intensity from AT-rich regions of the DNA. We thus obtain a DNA barcode that is dark in AT-rich regions and bright in GC-rich regions with kilobasepair resolution. We demonstrate the versatility of the method by obtaining a barcode on DNA from the phage T4 that captures its circular permutation and agrees well with its known sequence.

channels

DNA mapping

netropsin

restriction maps

dyes

Competitive assay

Nanofluidic. channels

resolution

double-stranded dna

distamycin

Single DNA

complexes

molecules

Fluorescence microscopy

Författare

Lena Nyberg

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Fredrik Persson

Chalmers, Fysik

Uppsala universitet

J. Berg

Chalmers, Kemi- och bioteknik

Johanna Bergström

Chalmers, Kemi- och bioteknik

Emelie Fransson

Chalmers, Kemi- och bioteknik

Lisbeth Olsson

Industriell bioteknik

Chalmers, Kemi- och bioteknik

M. Persson

Chalmers, Kemi- och bioteknik

Antti Stålnacke

Chalmers, Kemi- och bioteknik

Jens Wigenius

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

J. O. Tegenfeldt

Göteborgs universitet

Fredrik Westerlund

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Biochemical and Biophysical Research Communications

0006-291X (ISSN) 1090-2104 (eISSN)

Vol. 417 1 404-408

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik (2010-2018)

Ämneskategorier

Biokemi och molekylärbiologi

Kemi

DOI

10.1016/j.bbrc.2011.11.128

PubMed

22166208

Mer information

Senast uppdaterat

2022-02-08