Lipid-Based Passivation in Nanofluidics
Artikel i vetenskaplig tidskrift, 2012

Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.

passivation

single molecules

antifouling

Nanofluidics

protein−DNA interactions

lipid bilayer

Författare

Fredrik Persson

Göteborgs universitet

Joachim Fritzsche

Göteborgs universitet

K. U. Mir

Wellcome Trust Centre for Human Genetics

M. Modesti

Centre de Recherche en Cancerologie de Marseille

Fredrik Westerlund

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

J. O. Tegenfeldt

Göteborgs universitet

Nano Letters

1530-6984 (ISSN) 1530-6992 (eISSN)

Vol. 12 5 2260-2265

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

NATURVETENSKAP

TEKNIK OCH TEKNOLOGIER

DOI

10.1021/nl204535h

Mer information

Skapat

2017-10-07