An anomalous behavior of trypsin immobilized in alginate network
Artikel i vetenskaplig tidskrift, 2013

Alginate is a biopolymer used in drug formulations and for surgical purposes. In the presence of divalent cations, it forms solid gels, and such gels are of interest for immobilization of cells and enzymes. In this work, we entrapped trypsin in an alginate gel together with a known substrate, N (alpha)-benzoyl-l-arginine-4-nitroanilide hydrochloride (l-BAPNA), and in the presence or absence of d-BAPNA, which is known to be a competitive inhibitor. Interactions between alginate and the substrate as well as the enzyme were characterized with transmission electron microscopy, rheology, and nuclear magnetic resonance spectroscopy. The biocatalysis was monitored by spectrophotometry at temperatures ranging from 10 to 42 A degrees C. It was found that at 37 and 42 A degrees C a strong acceleration of the reaction was obtained, whereas at 10 A degrees C and at room temperature, the presence of d-BAPNA leads to a retardation of the reaction rate. The same effect was found when the reaction was performed in a non-cross-linked alginate solution. In alginate-free buffer solution, as well as in a solution of carboxymethylcellulose, a biopolymer that resembles alginate, the normal behavior was obtained; however, with d-BAPNA acting as an inhibitor at all temperatures. A more detailed investigation of the reaction kinetics showed that at higher temperature and in the presence of alginate, the curve of initial reaction rate versus l-BAPNA concentration had a sigmoidal shape, indicating an allosteric behavior. We believe that the anomalous behavior of trypsin in the presence of alginate is due to conformational changes caused by interactions between the positively charged trypsin and the strongly negatively charged alginate.

Trypsin

silica

Alginate

lipase

enzyme immobilization

stability

N-alpha-benzoyl-L-arginine-4-nitroanilide hydrochloride

chitosan beads

sol-gel transition

gelation

Immobilization

hydrogels

Allostery

Författare

C. Ganachaud

Chalmers

Diana Bernin

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

SuMo Biomaterials

Dan Isaksson

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Krister Holmberg

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Applied Microbiology and Biotechnology

0175-7598 (ISSN) 1432-0614 (eISSN)

Vol. 97 10 4403-4414

Ämneskategorier

Mikrobiologi

DOI

10.1007/s00253-012-4333-4

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Senast uppdaterat

2018-09-10