Self-calibrated concentration measurements of polydisperse nanoparticles
Artikel i vetenskaplig tidskrift, 2013

Summary Quantitative characterization of nanoparticles, e.g. accurate estimation of concentration distributions, is critical to many pharmaceutical and biological applications. We present a method that enables for the first time highly accurate size and absolute concentration measurements of polydisperse nanoparticles in solution, based on fluorescence single particle tracking, that are self-calibrated in the sense that the detection region volume is estimated based on the tracking data. The method is evaluated using simulations and experimental data of polystyrene nanospheres in water/sucrose solution. In addition, the method is used to quantify aggregation and clearance of different types of liposomes after intravenous injection in rats, where additional and more accurate information can be obtained that was previously unavailable, which can help elucidate their usefulness as drug carriers.

biological-fluids

Concentration

nanoparticles

polydispersity

size distribution

quantum dots

single-particle tracking

fluorescence single particle tracking

fluorescence

cells

liposomal drug carriers

Författare

Magnus Röding

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

SuMo Biomaterials

Hendrik Deschout

Universiteit Gent

Kevin Braeckmans

Universiteit Gent

Aila Särkkä

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

Mats Rudemo

Chalmers, Matematiska vetenskaper, matematisk statistik

Göteborgs universitet

Journal of Microscopy

0022-2720 (ISSN) 1365-2818 (eISSN)

Vol. 252 79-88

Ämneskategorier

Nanoteknik

DOI

10.1111/jmi.12073