Self-calibrated concentration measurements of polydisperse nanoparticles
Journal article, 2013

Summary Quantitative characterization of nanoparticles, e.g. accurate estimation of concentration distributions, is critical to many pharmaceutical and biological applications. We present a method that enables for the first time highly accurate size and absolute concentration measurements of polydisperse nanoparticles in solution, based on fluorescence single particle tracking, that are self-calibrated in the sense that the detection region volume is estimated based on the tracking data. The method is evaluated using simulations and experimental data of polystyrene nanospheres in water/sucrose solution. In addition, the method is used to quantify aggregation and clearance of different types of liposomes after intravenous injection in rats, where additional and more accurate information can be obtained that was previously unavailable, which can help elucidate their usefulness as drug carriers.

liposomal drug carriers

polydispersity

fluorescence single particle tracking

biological-fluids

size distribution

Concentration

nanoparticles

quantum dots

cells

fluorescence

single-particle tracking

Author

Magnus Röding

University of Gothenburg

SuMo Biomaterials

Chalmers, Mathematical Sciences, Mathematical Statistics

Hendrik Deschout

Ghent university

Kevin Braeckmans

Ghent university

Aila Särkkä

University of Gothenburg

Chalmers, Mathematical Sciences, Mathematical Statistics

Mats Rudemo

Chalmers, Mathematical Sciences, Mathematical Statistics

University of Gothenburg

Journal of Microscopy

0022-2720 (ISSN) 1365-2818 (eISSN)

Vol. 252 1 79-88

Subject Categories

Nano Technology

DOI

10.1111/jmi.12073

More information

Latest update

8/18/2020