Mapping Condition-Dependent Regulation of Lipid Metabolism in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2013

Lipids play a central role in cellular function as constituents of membranes, as signaling molecules, and as storage materials. Although much is known about the role of lipids in regulating specific steps of metabolism, comprehensive studies integrating genome-wide expression data, metabolite levels, and lipid levels are currently lacking. Here, we map condition-dependent regulation controlling lipid metabolism in Saccharomyces cerevisiae by measuring 5636 mRNAs, 50 metabolites, 97 lipids, and 57 C-13-reaction fluxes in yeast using a three-factor full-factorial design. Correlation analysis across eight environmental conditions revealed 2279 gene expression level-metabolite/lipid relationships that characterize the extent of transcriptional regulation in lipid metabolism relative to major metabolic hubs within the cell. To query this network, we developed integrative methods for correlation of multi-omics datasets that elucidate global regulatory signatures. Our data highlight many characterized regulators of lipid metabolism and reveal that sterols are regulated more at the transcriptional level than are amino acids. Beyond providing insights into the systems-level organization of lipid metabolism, we anticipate that our dataset and approach can join an emerging number of studies to be widely used for interrogating cellular systems through the combination of mathematical modeling and experimental biology.

yeast

phospholipid-synthesis

lipid metabolism

omics

metabolome

escherichia-coli

sterol synthesis

chemostat

regulation

fatty-acid synthesis

growth temperature

transcriptional

integrated systems biology

data sets

cultures

regulation

genome-reduced bacterium

Författare

M. C. Jewett

Northwestern University

Danmarks Tekniske Universitet (DTU)

C. T. Workman

Danmarks Tekniske Universitet (DTU)

Intawat Nookaew

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

F. A. Pizarro

Pontificia Universidad Catolica de Chile

Danmarks Tekniske Universitet (DTU)

E. Agosin

Pontificia Universidad Catolica de Chile

Danmarks Tekniske Universitet (DTU)

L. I. Hellgren

Danmarks Tekniske Universitet (DTU)

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

G3: Genes, Genomes, Genetics

2160-1836 (ISSN)

Vol. 3 11 1979-1995

Ämneskategorier

Genetik

DOI

10.1534/g3.113.006601

Mer information

Senast uppdaterat

2018-05-28