Nanomedical Relevance of the Intermolecular Interaction Dynamics-Examples from Lysozymes and Insulins
Artikel i vetenskaplig tidskrift, 2019
Insulin and lysozyme share the common features of being prone to aggregate and having biomedical importance. Encapsulating lysozyme and insulin in micellar nanoparticles probably would prevent aggregation and facilitate oral drug delivery. Despite the vivid structural knowledge of lysozyme and insulin, the environment-dependent oligomerization (dimer, trimer, and multimer) and associated structural dynamics remain elusive. The knowledge of the intra- and intermolecular interaction profiles has cardinal importance for the design of encapsulation protocols. We have employed various biophysical methods such as NMR spectroscopy, X-ray crystallography, Thioflavin T fluorescence, and atomic force microscopy in conjugation with molecular modeling to improve the understanding of interaction dynamics during homo-oligomerization of lysozyme (human and hen egg) and insulin (porcine, human, and glargine). The results obtained depict the atomistic intra- and intermolecular interaction details of the homo-oligomerization and confirm the propensity to form fibrils. Taken together, the data accumulated and knowledge gained will further facilitate nanoparticle design and production with insulin or lysozyme-related protein encapsulation.
Författare
Ruiyan Zhang
Christian-Albrechts-Universität zu Kiel
RI-B-NT Research Institute of Bioinformatics and Nanotechnology
Liaocheng University
Ning Zhang
Liaocheng University
Marzieh Mohri
RI-B-NT Research Institute of Bioinformatics and Nanotechnology
Lisha Wu
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Thomas Eckert
Hochschule Fresenius
Justus-Liebig-Universität Gießen
Vadim B. Krylov
Russian Academy of Sciences
Andrea Antosova
Slovak Academy of Sciences
Slavomira Ponikova
Slovak Academy of Sciences
Zuzana Bednarikova
Slovak Academy of Sciences
Philipp Markart
Klinikum Fulda
Justus-Liebig-Universität Gießen
Andreas Guenther
Russian Academy of Sciences
Justus-Liebig-Universität Gießen
Bengt Nordén
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Martin Billeter
Göteborgs universitet
Roland Schauer
Christian-Albrechts-Universität zu Kiel
Axel J. Scheidig
Christian-Albrechts-Universität zu Kiel
Bhisma N. Ratha
Bose Institute
Anirban Bhunia
Bose Institute
Karsten Hesse
Tierarztpraxis Dr. Karsten Hesse
Mushira Abdelaziz Enani
King Fahad Medical City
Juergen Steinmeyer
Justus-Liebig-Universität Gießen
Athanasios K. Petridis
Heinrich Heine Universität Düsseldorf
Tibor Kozar
Univerzita Pavla Jozefa Šafárika
Zuzana Gazova
Slovak Academy of Sciences
Hans-Christian Siebert
RI-B-NT Research Institute of Bioinformatics and Nanotechnology
ACS Omega
24701343 (eISSN)
Vol. 4 2 4206-4220Ämneskategorier
Fysikalisk kemi
Annan kemi
Biofysik
DOI
10.1021/acsomega.8b02471
PubMed
30847433