Comparison of strategies to overcome the inhibitory effects in high-gravity fermentation of lignocellulosic hydrolysates
Artikel i vetenskaplig tidskrift, 2014

High-gravity (HG) technology aims at generating final ethanol concentrations above 50 kg m(-3) in order to reduce the cost of the distillation step. The generation of higher amounts of inhibitors during the pretreatment step is one of the challenges that accompany the increase in initial dry matter. Detoxification of spruce hydrolysate, adaptation of the cells before fermentation, supplementation with nutrients, and washing of solids were the strategies compared in this study. They represent different approaches to cope with the inhibitory effects, and we compared their efficiencies using a thermotolerant strain of Saccharomyces cereuisiae at temperatures from 30 degrees C up to 40 degrees C. The dilute acid-pretreated spruce used as substrate in this study was not fermentable under HG conditions (200 g kg(-1) water-insoluble solids) when no improvement method was applied. In HG simultaneous saccharification and fermentation at 30 degrees C combined with a 24 h pre-hydrolysis step, the detoxification of pretreated spruce with reducing agent (Na2S2O4) gave the best result with an ethanol yield of 57% (on total sugars) of the maximum theoretical and a volumetric productivity of 1.58 g dm(-3) h(-1). In HG separate hydrolysis and fermentation, nutrients supplementation gave better final ethanol yields than detoxification of the material, reaching an ethanol yield of about 60% of the theoretical (on total sugars). The results obtained, showed an increase in severity of inhibitory effects with temperature increase. Improved cell viability was observed when detoxified material was used and also when yeast extract addition was coupled with adaptation of the cells to the hydrolysate. (C) 2014 Published by Elsevier Ltd.

STEAM PRETREATMENT

DETOXIFICATION

REDUCING AGENTS

EELS EL

MICROBIOLOGY-UK

Biotechnology & Applied Microbiology

Lignocellulosic inhibitors

BIOETHANOL PRODUCTION

1994

Softwood

Nutrient supplementation

WHEAT-STRAW

Energy &

Agricultural Engineering

V140

ACETIC-ACID

ETHANOL-PRODUCTION

ENZYMATIC-HYDROLYSIS

GROWTH

Bioethanol

Fuels

Detoxification

SACCHAROMYCES-CEREVISIAE

P569

Författare

Charilaos Xiros

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Biomass and Bioenergy

0961-9534 (ISSN)

Vol. 65 79-90

Ämneskategorier

Biokemi och molekylärbiologi

Styrkeområden

Energi

DOI

10.1016/j.biombioe.2014.03.060

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2017-10-07