Nanosized fibers' effect on adult human articular chondrocytes behavior.
Artikel i vetenskaplig tidskrift, 2013

Tissue engineering with chondrogenic cell based therapies is an expanding field with the intention of treating cartilage defects. It has been suggested that scaffolds used in cartilage tissue engineering influence cellular behavior and thus the long-term clinical outcome. The objective of this study was to assess whether chondrocyte attachment, proliferation and post-expansion re-differentiation could be influenced by the size of the fibers presented to the cells in a scaffold. Polylactic acid (PLA) scaffolds with different fiber morphologies were produced, i.e. microfiber (MS) scaffolds as well as nanofiber-coated microfiber scaffold (NMS). Adult human articular chondrocytes were cultured in the scaffolds in vitro up to 28 days, and the resulting constructs were assessed histologically, immunohistochemically, and biochemically. Attachment of cells and serum proteins to the scaffolds was affected by the architecture. The results point toward nano-patterning onto the microfibers influencing proliferation of the chondrocytes, and the overall 3D environment having a greater influence on the re-differentiation. In the efforts of finding the optimal scaffold for cartilage tissue engineering, studies as the current contribute to the knowledge of how to affect and control chondrocytes behavior.

ultrastructure

Immunohistochemistry

Cell Shape

Cell Count

Articular

Tissue Scaffolds

Nanofibers

Chondrocytes

Cartilage

Particle Size

cytology

cytology

Humans

chemistry

Cell Adhesion

Adult

Chondrogenesis

Cell Differentiation

ultrastructure

Cell Proliferation

Författare

Hanna Stenhamre

Chalmers, Kemi- och bioteknik, Polymerteknologi

Anna Thorvaldsson

Chalmers, Kemi- och bioteknik, Polymerteknologi

Lars Enochson

Göteborgs universitet

Pernilla Walkenström

Swerea IVF AB

Anders Lindahl

Göteborgs universitet

Mats Brittberg

Göteborgs universitet

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Materials science & engineering. C, Materials for biological applications

1873-0191 (ISSN)

Vol. 33 1539-1545

Ämneskategorier

Materialteknik

Polymerteknologi

DOI

10.1016/j.msec.2012.12.059

PubMed

23827606