Industrial yeasts strains for biorefinery solutions: Constructing and selecting efficient barcoded xylose fermenting strains for ethanol
Artikel i vetenskaplig tidskrift, 2014

There have been intense efforts in the scientific community and industry to engineer S. cerevisiae strains to co-consume xylose and glucose. In this context, this paper describes a process for introducing a barcode into an industrial xylose fermenting yeast strains to ensure quick and reliable identification of patented industrial strains. Furthermore, this work presents a novel procedure for screening and selecting the clones that reflects the characteristics of the industrial process. The key points of efficient selection of high-performing clones are elaborated and discussed. In spite of the unforeseen effects on yeast physiology after the introduction of a new sequence into the genome, we obtained barcoded clones with the same xylose consumption rates and ethanol yields as the cells without the barcode.

lignocellulosic biomass

saccharomyces-cerevisiae

pluripotential cells

Författare

Elia Tomas-Pejo

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Nicklas Bonander

Taurus energy

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Biofuels, Bioproducts and Biorefining

1932-104X (ISSN)

Vol. 8 5 626-634

Ämneskategorier

Bioenergi

DOI

10.1002/bbb.1472