Altered sterol composition renders yeast thermotolerant
Artikel i vetenskaplig tidskrift, 2014

Ethanol production for use as a biofuel is mainly achieved through simultaneous saccharification and fermentation by yeast. Operating at >/=40 degrees C would be beneficial in terms of increasing efficiency of the process and reducing costs, but yeast does not grow efficiently at those temperatures. We used adaptive laboratory evolution to select yeast strains with improved growth and ethanol production at >/=40 degrees C. Sequencing of the whole genome, genome-wide gene expression, and metabolic-flux analyses revealed a change in sterol composition, from ergosterol to fecosterol, caused by mutations in the C-5 sterol desaturase gene, and increased expression of genes involved in sterol biosynthesis. Additionally, large chromosome III rearrangements and mutations in genes associated with DNA damage and respiration were found, but contributed less to the thermotolerant phenotype

Författare

Luis Caspeta-Guadarrama

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Yun Chen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

PAYAM GHIACI

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Amir Feizi

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Steen Buskov

Novozymes AS

B. M. Hallstrom

Kungliga Tekniska Högskolan (KTH)

Dina Petranovic Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Science

0036-8075 (ISSN)

Vol. 346 75-78

Ämneskategorier

Biologiska vetenskaper

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1126/science.1258137