Altered sterol composition renders yeast thermotolerant
Journal article, 2014

Ethanol production for use as a biofuel is mainly achieved through simultaneous saccharification and fermentation by yeast. Operating at >/=40 degrees C would be beneficial in terms of increasing efficiency of the process and reducing costs, but yeast does not grow efficiently at those temperatures. We used adaptive laboratory evolution to select yeast strains with improved growth and ethanol production at >/=40 degrees C. Sequencing of the whole genome, genome-wide gene expression, and metabolic-flux analyses revealed a change in sterol composition, from ergosterol to fecosterol, caused by mutations in the C-5 sterol desaturase gene, and increased expression of genes involved in sterol biosynthesis. Additionally, large chromosome III rearrangements and mutations in genes associated with DNA damage and respiration were found, but contributed less to the thermotolerant phenotype

Author

Luis Caspeta-Guadarrama

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Yun Chen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

PAYAM GHIACI

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Amir Feizi

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Steen Buskov

Novozymes A/S

B. M. Hallstrom

Royal Institute of Technology (KTH)

Dina Petranovic Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Science

0036-8075 (ISSN)

Vol. 346 6205 75-78

Subject Categories

Biological Sciences

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1126/science.1258137

More information

Latest update

7/3/2018 1