Enhanced amino acid utilization sustains growth of cells lacking Snf1/AMPK
Artikel i vetenskaplig tidskrift, 2015

The metabolism of proliferating cells shows common features even in evolutionary distant organisms such as mammals and yeasts, for example the requirement for anabolic processes under tight control of signaling pathways. Analysis of the rewiring of metabolism, which occurs following the dysregulation of signaling pathways, provides new knowledge about the mechanisms underlying cell proliferation. The key energy regulator in yeast Snf1 and its mammalian ortholog AMPK have earlier been shown to have similar functions at glucose limited conditions and here we show that they also have analogies when grown with glucose excess. We show that loss of Snf1 in cells growing in 2% glucose induces an extensive transcriptional reprogramming, enhances glycolytic activity, fatty add accumulation and reliance on amino acid utilization for growth. Strikingly, we demonstrate that Snf1/AMPK-deficient cells remodel their metabolism fueling mitochondria and show glucose and amino acids addiction, a typical hallmark of cancer cells.

Glucose

Metabolism

Saccharomyces cerevisiae

Respiration

Budding yeast

Gene chip

Författare

Raffaele Nicastro

Universita' degli Studi di Milano-Bicocca

Farida Tripodi

Universita' degli Studi di Milano-Bicocca

C. Guzzi

Universita' degli Studi di Milano-Bicocca

V. Reghellin

Universita' degli Studi di Milano-Bicocca

Sakda Khoomrung

Chalmers, Biologi och bioteknik, Systembiologi

C. Capusoni

Universita degli Studi di Milano

C. Compagno

Universita degli Studi di Milano

C. Airoldi

Universita' degli Studi di Milano-Bicocca

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Lilia Alberghina

Universita' degli Studi di Milano-Bicocca

Paola Coccetti

Universita' degli Studi di Milano-Bicocca

Biochimica et Biophysica Acta - Molecular Cell Research

0167-4889 (ISSN)

Vol. 1853 1615-1625

Ämneskategorier

Biokemi och molekylärbiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1016/j.bbamcr.2015.03.014