Enhanced amino acid utilization sustains growth of cells lacking Snf1/AMPK
Journal article, 2015

The metabolism of proliferating cells shows common features even in evolutionary distant organisms such as mammals and yeasts, for example the requirement for anabolic processes under tight control of signaling pathways. Analysis of the rewiring of metabolism, which occurs following the dysregulation of signaling pathways, provides new knowledge about the mechanisms underlying cell proliferation. The key energy regulator in yeast Snf1 and its mammalian ortholog AMPK have earlier been shown to have similar functions at glucose limited conditions and here we show that they also have analogies when grown with glucose excess. We show that loss of Snf1 in cells growing in 2% glucose induces an extensive transcriptional reprogramming, enhances glycolytic activity, fatty add accumulation and reliance on amino acid utilization for growth. Strikingly, we demonstrate that Snf1/AMPK-deficient cells remodel their metabolism fueling mitochondria and show glucose and amino acids addiction, a typical hallmark of cancer cells.

Glucose

Metabolism

Saccharomyces cerevisiae

Respiration

Budding yeast

Gene chip

Author

Raffaele Nicastro

University of Milano-Bicocca

Farida Tripodi

University of Milano-Bicocca

C. Guzzi

University of Milano-Bicocca

V. Reghellin

University of Milano-Bicocca

Sakda Khoomrung

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

C. Capusoni

University of Milan

C. Compagno

University of Milan

C. Airoldi

University of Milano-Bicocca

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Lilia Alberghina

University of Milano-Bicocca

Paola Coccetti

University of Milano-Bicocca

Biochimica et Biophysica Acta - Molecular Cell Research

0167-4889 (ISSN)

Vol. 1853 7 1615-1625

Subject Categories

Biochemistry and Molecular Biology

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1016/j.bbamcr.2015.03.014

More information

Latest update

2/26/2018