The gut microbiota modulates host amino acid and glutathione metabolism in mice
Artikel i vetenskaplig tidskrift, 2015

The gut microbiota has been proposed as an environmental factor that promotes the progression of metabolic diseases. Here, we investigated how the gut microbiota modulates the global metabolic differences in duodenum, jejunum, ileum, colon, liver, and two white adipose tissue depots obtained from conventionally raised (CONV-R) and germ-free (GF) mice using gene expression data and tissue-specific genome-scale metabolic models (GEMs). We created a generic mouse metabolic reaction (MMR) GEM, reconstructed 28 tissue-specific GEMs based on proteomics data, and manually curated GEMs for small intestine, colon, liver, and adipose tissues. We used these functional models to determine the global metabolic differences between CONV-R and GF mice. Based on gene expression data, we found that the gut microbiota affects the host amino acid (AA) metabolism, which leads to modifications in glutathione metabolism. To validate our predictions, we measured the level of AAs and N-acetylated AAs in the hepatic portal vein of CONV-R and GF mice. Finally, we simulated the metabolic differences between the small intestine of the CONV-R and GF mice accounting for the content of the diet and relative gene expression differences. Our analyses revealed that the gut microbiota influences host amino acid and glutathione metabolism in mice.

metabolomics

transcriptomics

genome-scale metabolic models

germ-free mice

glutathione metabolism

Författare

Adil Mardinoglu

Chalmers, Biologi och bioteknik, Systembiologi

Saeed Shoaie

Chalmers, Biologi och bioteknik, Systembiologi

Mattias Bergentall

Göteborgs universitet

Pouyan Ghaffari Nouran

Chalmers, Biologi och bioteknik, Systembiologi

C. Zhang

Kungliga Tekniska Högskolan (KTH)

E. G. Larsson

Göteborgs universitet

Fredrik Bäckhed

Göteborgs universitet

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Molecular Systems Biology

1744-4292 (ISSN)

Vol. 11 Article Number: 834- 834

Metagenomics in Cardiometabolic Diseases (METACARDIS)

Europeiska kommissionen (FP7), 2012-11-01 -- 2017-10-31.

Ämneskategorier

Biokemi och molekylärbiologi

Cell- och molekylärbiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.15252/msb.20156487

PubMed

26475342