Rapid Tracing of Resistance Plasmids in a Nosocomial Outbreak Using Optical DNA Mapping
Artikel i vetenskaplig tidskrift, 2016

Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum beta-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.

spectrum beta-lactamases

optical DNA mapping

plasmids

Pharmacology & Pharmacy

enterobacteriaceae

field gel-electrophoresis

escherichia-coli

spread

molecules

nosocomial outbreak

antibiotic resistance

sequence

Infectious Diseases

antibiotic-resistance

Författare

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

N. Karami

Göteborgs universitet

Lena Nyberg

Chalmers, Biologi och bioteknik, Kemisk biologi

C. Pichler

Lunds universitet

P. C. T. Pedreschi

Lunds universitet

Mahmood Saair Quaderi

Chalmers, Biologi och bioteknik

Joachim Fritzsche

Chalmers, Fysik, Kemisk fysik

T. Ambjornsson

Lunds universitet

C. Ahren

Göteborgs universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

ACS Infectious Diseases

2373-8227 (eISSN)

Vol. 2 5 322-328

Ämneskategorier

Klinisk medicin

DOI

10.1021/acsinfecdis.6b00017