FRET enhancement close to gold nanoparticles positioned in DNA origami constructs
Artikel i vetenskaplig tidskrift, 2017

Here we investigate the energy transfer rates of a Förster resonance energy transfer (FRET) pair positioned in close proximity to a 5 nm gold nanoparticle (AuNP) on a DNA origami construct. We study the distance dependence of the FRET rate by varying the location of the donor molecule, D, relative to the AuNP while maintaining a fixed location of the acceptor molecule, A. The presence of the AuNP induces an alteration in the spontaneous emission of the donor (including radiative and non-radiative rates) which is strongly dependent on the distance between the donor and AuNP surface. Simultaneously, the energy transfer rates are enhanced at shorter D-A (and D-AuNP) distances. Overall, in addition to the direct influence of the acceptor and AuNP on the donor decay there is also a significant increase in decay rate not explained by the sum of the two interactions. This leads to enhanced energy transfer between donor and acceptor in the presence of a 5 nm AuNP. We also demonstrate that the transfer rate in the three "particle" geometry (D + A + AuNP) depends approximately linearly on the transfer rate in the donor-AuNP system, suggesting the possibility to control FRET process with electric field induced by 5 nm AuNPs close to the donor fluorophore. It is concluded that DNA origami is a very versatile platform for studying interactions between molecules and plasmonic nanoparticles in general and FRET enhancement in particular.

Energy transfer

Metal nanoparticles

Gold

Electric fields

Nanoparticles

Molecules

Författare

Nesrine Aissaoui

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Kasper Moth-Poulsen

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Polymerteknologi

Mikael Käll

Chalmers, Fysik, Bionanofotonik

Peter Johansson

Örebro universitet

Marcus Wilhelmsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Bo Albinsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Nanoscale

2040-3364 (ISSN)

Vol. 9 673-683

Styrkeområden

Nanovetenskap och nanoteknik

Ämneskategorier

Fysikalisk kemi

DOI

10.1039/c6nr04852h