Extracellular vesicles from human pancreatic islets suppress human islet amyloid polypeptide amyloid formation
Artikel i vetenskaplig tidskrift, 2017

Extracellular vesicles (EVs) are small vesicles released by cells to aid cell-cell communication and tissue homeostasis. Human islet amyloid polypeptide (IAPP) is the major component of amyloid deposits found in pancreatic islets of patients with type 2 diabetes (T2D). IAPP is secreted in conjunction with insulin from pancreatic beta cells to regulate glucose metabolism. Here, using a combination of analytical and biophysical methods in vitro, we tested whether EVs isolated from pancreatic islets of healthy patients and patients with T2D modulate IAPP amyloid formation. We discovered that pancreatic EVs from healthy patients reduce IAPP amyloid formation by peptide scavenging, but T2D pancreatic and human serum EVs have no effect. In accordance with these differential effects, the insulin: C-peptide ratio and lipid composition differ between EVs from healthy pancreas and EVs from T2D pancreas and serum. It appears that healthy pancreatic EVs limit IAPP amyloid formation via direct binding as a tissue-specific control mechanism.

amyloid

type 2 diabetes

extracellular vesicles

electron microscopy

atomic force microscopy

Författare

Diana Ribeiro

Chalmers, Biologi och bioteknik, Kemisk biologi

Istvan Horvath

Chalmers, Biologi och bioteknik, Kemisk biologi

N. Heath

AstraZeneca

R. Hicks

AstraZeneca Sweden

A. Forslow

AstraZeneca Sweden

Pernilla Wittung Stafshede

Chalmers, Biologi och bioteknik, Kemisk biologi

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 114 11127-11132

Ämneskategorier

Cellbiologi

DOI

10.1073/pnas.1711389114

PubMed

28973954