Fungal Glucuronoyl and Feruloyl Esterases for Wood Processing and Phenolic Acid Ester/Sugar Ester Synthesis
Poster (konferens), 2015
Feruloyl esterases (FAEs, E.C. 22.214.171.124, CAZy family CE1) and glucuronoyl esterases
(GEs, E.C. 3.1.1.-, CAZy family CE15) are involved in the degradation of plant
biomass by hydrolysing ester linkages in plant cell walls, and thus have potential use in
biofuel production from lignocellulosic materials and in biorefinery applications with
the aim of developing new wood-based compounds [1, 2]. GEs and FAEs are present in
the genomes of a wide range of fungi and bacteria.
Under conditions of low water content, these enzymes can also carry out
(trans)esterification reactions, making them promising biocatalysts for the modification
of compounds with applications in the food, cosmetic and pharmaceutical industry.
Compared to the chemical process, enzymatic synthesis can be carried out under lower
process temperatures (50-60°C) and results in fewer side products, thus reducing the
We characterised new FAE and GE enzymes from mesophilic, thermophilic and coldtolerant
filamentous fungi produced in Pichia pastoris. The enzymes were characterised
for both their hydrolytic abilities on various model substrates (methyl ferulate, pNPferulate)
- for potential applications in deconstruction of lignocellulosic materials and
extraction of valuable compounds - as well as for their biosynthetic capacities. We
tested and optimised the FAEs’ transesterification capabilities on ferulate esters in a 1-
butanol-buffer system, with the aim of using the most promising candidates for the
production of antioxidant compounds with improved hydrophobic or hydrophilic
properties, such as prenyl ferulate, prenyl caffeate, glyceryl ferulate and 5-O-(transferuloyl)-arabinofuranose.