Plasma membrane as a crucial player in acetic acid effect on yeast
Övrigt konferensbidrag, 2017

Weak organic acids such as formic, acetic or lactic acid are known inhibitors of microbial growth and fermentation. Acetic acid toxicity to yeast cells has been explained by different theories, involving specific signaling effects triggering an active cell death program, reduction of intracellular pH and acetate anion accumulation. Regardless of the fact whether the actual effect of acetate involves one of these mechanisms or a combination thereof, acetic acid inhibits yeast metabolism and affects yeast viability. This has a high impact on the feasibility of the new generation of fermentation processes, based on the naturally acetic acid-rich lignocellulosic substrates. It is therefore highly desirable to obtain a strain with increased capacity of coping with high acetic acid concentrations in the fermentation medium. Acetic acid is thought to be internalized by yeast cells in its undissociated form, by crossing the hydrophobic barrier of plasma membrane. Thus, in our work we focused on the investigation of membrane properties and how these influence the tolerance of yeast to acetic acid. First, we demonstrated with lipidomics analysis of membrane lipids that the yeast Zygosaccharomyces bailii, showing extraordinary tolerance to acetic acid, has a plasma membrane which is rich in sphingolipids. Next, we combined membrane molecular dynamics and in vivo measurements to confirm the specific role of sphingolipids in altering the permeability of plasma membrane to acetic acid. Finally, we investigated the effect of alcohols on the acetic acid permeation rate through the membrane. Our ultimate goal is to engineer the membrane composition of an industrial yeast strain towards reduced permeability, in order to obtain higher acetic acid tolerance.


Maurizio Bettiga

Chalmers, Biologi och bioteknik, Industriell bioteknik

Lina Lindahl

Chalmers, Biologi och bioteknik, Industriell bioteknik

Samuel Genheden

Göteborgs universitet

Leif A Eriksson

Göteborgs universitet

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

IMYA12- 12th International Meeting on Yeast Apoptosis, Bari, Italy • 14-18 May 2017


Hållbar utveckling


Industriell bioteknik

Biokemi och molekylärbiologi

Biologiska vetenskaper




Livsvetenskaper och teknik (2010-2018)


Grundläggande vetenskaper

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