Lactic acid decreases Fe (II) and Fe (III) retention but increases Fe (III) transepithelial transfer by Caco-2 cells
Artikel i vetenskaplig tidskrift, 2005

Lactic acid (LA) has been proposed to be an enhancer for dietary iron absorption, but contradictory results have also been reported. In the present study, fully differentiated Caco-2 cell monolayers were used to evaluate the effects of LA (1-50 mmol/L) on the cellular retention and transepithelial transport of soluble non-heme iron (as ferric nitrilotriacetate). Our data revealed a linear decline in Fe(III) retention with respect to the concentration of LA added. In the presence of 50 mmol/L LA, retention of Fe(111) and Fe(II) decreased 57% and 58%, respectively. In contrast, transfer of Fe(111) across the cell monolayer was doubled, while Fe(II) transfer across the cell monolayer decreased 35%. We conclude that LA reduces cellular retention and transepithelial transport of Fe(l I) by Caco-2 cells in a dose-dependent manner. However, while LA also reduces retention of Fe(111) by Caco-2 cells, the transfer of Fe(III) across cell monolayers is enhanced, possibly due to effects on paracellular transport.

Författare

Sharon W Bergkvist

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Ann-Sofie Sandberg

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Thomas Andlid

Chalmers, Kemi- och bioteknik, Livsvetenskaper

M Wessling-Resnick

Harvard School of Public Health

Publicerad i

Journal of Agricultural and Food Chemistry

0021-8561 (ISSN) 1520-5118 (eISSN)

Vol. 53 Nummer/häfte 17 s. 6919-6923

Kategorisering

Ämneskategorier (SSIF 2011)

Livsmedelsvetenskap

Identifikatorer

DOI

10.1021/jf050892s

Mer information

Skapat

2017-10-07